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Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study.

Authors: Bareh GM, Jacoby E, Binkley P, Chang TC, Schenken RS, Robinson RD.
Publication date: 20170116
Journal: Fertil Steril. 2016 Feb;105(2):329-36.e1. Epub 2015 Nov 19.
DOI: 10.1016/j.fertnstert.2015.10.033.

OBJECTIVE:

To determine whether sperm DNA integrity in normozoospermic male partners plays a role in idiopathic recurrent pregnancy loss (RPL).

DESIGN:

Prospective, cohort study.

SETTING:

Academic tertiary care center.

PATIENT(S):

Group I: 26 male partners of women with unexplained RPL. Group II: 31 normozoospermic males with proven fertility.

INTERVENTION(S):

Semen samples were collected by masturbation after 48-72 hours of abstinence. After liquefaction at room temperature, semen analysis was performed according to World Health Organization standards. Only samples with >20 × 10(6) spermatozoa/mL with at least 50% progressive sperm motility and 30 % normal morphology were selected for the study. DNA fragmentation of the sperm was assessed with TUNEL assay followed by flow cytometric analysis.

MAIN OUTCOME MEASURE(S):

Sperm DNA fragmentation in both groups.

RESULT(S):

Mean DNA fragmentation (mean ± SD) was significantly more in men with RPL (36.8 ± 5) compared with controls (9.4 ± 2.7).

CONCLUSION(S):

Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.

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